Coding

Part:BBa_K4834002:Design

Designed by: Julian Borbeck   Group: iGEM23_Tuebingen   (2023-10-11)


Potential Galactose 2 oxidizing Enzyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 400
    Illegal BamHI site found at 951
    Illegal BamHI site found at 1307
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Untested in the lab, enzyme needs copper cofactor, which is best added to the medium during induction of this enzyme.


Source

Uniprot ID: A0A178E5X4, Organism: Pyrenochaeta sp. DS3sAY3a

References